In order to screen out the effective fungicides and application methods to control peanut leaf spot disease,the effect of different fungicides on peanut leaf spot disease control and yield was studied by different spraying frequency and concentration. The results showed that the control efficiency different fungicide and treatment were between 28.44% and 55.06%. Baitai reached the best results of peanut leaf spot disease control. With spraying twice（15 days interval） diluted liquid of 1/600, the control efficiency reached 44.66%, and that of spraying three times（10days interval） was 55.06%. Spraying appropriate fungicides could effectively prolong leaf function and improve peanut yield. After using Baitai fungicides on peanut, the yield of pods and seeds increased 5.07%-16.20% and 6.13%-24.68%, respectively. The highest yield was obtained by spraying diluted liquid of 1/600 Baitai solution three times（10 days interval）. In general, the effect of Baitai on leaf spot disease was better than Benzopyrimidine ester and carbendazim. The effect of applying three times（10 days interval） was superior to spraying twice（15 days interval） and the effect of spraying with higher concentration was better than lower concentration.