The pea aphid is not merely one of important pest on crops, but it is also an ideal material for studying biological evolution mechanisms due to its complex life cycle and multiple reproductive types. In this paper, evaluated the effectiveness of leaf-disc method using Acyrthosiphon pisum and its host plant Vicia faba, to bring about a great advance in research of control and evolution of A. pisum. The V. faba leaf was cultured in leaf-disc method with 3 different kinds of nutrient solutions（Hyponex, Dewoduo and Compo）. It can be seen that Hyponex was the most suitable nutrient solution formula for V. faba leaf cultivation under this experimental condition. On the other hand, no significant difference was found in fecundity, longevity, body size and developmental time between leaf-disc method and V.faba seedling. Therefore, the present study suggests that leaf-disc method can be applied to rearing of pea aphid.