Nitrogen nutritional status is an important parameter for crop growth,and how to accurately monitor it is particularly important.In this paper,the winter wheat leaf reflection spectrum data and the corresponding leaf nitrogen content and leaf nitrogen accumulation data at the experimental base of Beijing Academy of Agricultural and Forestry Sciences in 2013-2014 were used to screen out the wave bands which are sensitive to leaf nitrogen content and leaf nitrogen accumulation by the combination of bands,and sensitive wave bands were used to establish nitrogen nutrition diagnosis model of winter wheat in each growth period and whole growth period.The results show that:（1） The sensitive spectral parameters of leaf nitrogen content and leaf nitrogen accumulation screened by the combination of bands are NDSI_{（564,728）},NDSI_{（543,728）},RSI_{（564,728）}and RSI_{（543,728）};（2） Among the nitrogen nutrition diagnosis models constructed in each growth period and the whole growth period,the stability and reliability of LNC in each growth period is better than LNA,and the leave-one-out cross validation method also shows that the accuracy of LNC is relatively higher.This study has shown that the nitrogen content of leaves can be used to monitor the nitrogen nutrition status of winter wheat,so as to achieve precise management of nitrogen fertilizer.