云南主栽蓝莓果实可滴定酸及抗氧化活性评价研究
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S663.9

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云南省高校联合基金面上项目(2017FH001-040)


Evaluation of Titratable Acid and Antioxidant Activity of Blueberry Fruits Grown in Yunnan
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    摘要:

    以云南主产区的6个品种蓝莓鲜果为研究对象,采用生理生化试验方法,比较不同品种蓝莓成熟果实提取液的可滴定酸、类黄酮、总酚、总花色苷含量及对其体外抗氧化活性进行评价。试验结果表明,6个品种中,比洛克西的可滴定酸含量、类黄酮含量、总酚含量、总花色苷含量最高,分别比夏普蓝高47.4%、111.81%、121.16%和2556.50%;体外抗氧化活性评价结果显示,比洛克西对DPPH自由基的清除能力最强,当浓度为0.8 mg/mL时,清除能力为56.8%;对ABTS+自由基的清除能力最好,浓度为1.0 mg/mL时,清除能力为88.7%;对OH-自由基的清除能力最高,当浓度为1.0 mg/mL时,清除能力为90.4%;6个品种蓝莓果实类黄酮、总酚、总花色苷含量与DPPH自由基、ABTS+自由基和OH-自由基清除率间均存在正相关关系,且相关性均达到极显著水平(P<0.01)。综上可知,比洛克西蓝莓果实可滴定酸含量较高,且抗氧化能力比其他5个品种强,品质优良,适宜进行综合加工和高值化利用。

    Abstract:

    The contents of titratable acids, flavonoids, total phenols, and total anthocyanins in the extracts of mature blueberry fruits from 6 varieties of fresh blueberry fruits in the main production areas of Yunnan Province were compared by physiological and biochemical methods, and their antioxidant activities in vitro were evaluated. The results showed that Biloxi had the highest titratable acid content, flavonoids content, total phenol content and total anthocyanin content, which were 47.4%, 111.81%, 121.16% and 2556.50% higher than Sharpblue. The results of antioxidant activity evaluation in vitro showed that Biloxi had the strongest scavenging capacity for DPPH free radical.When the concentration was 0.8 mg/mL, the scavenging capacity was 56.8%. The scavenging capacity of ABTS+was the best, and the scavenging capacity was 90.4% when the concentration was 1.0 mg/mL. The scavenging capacity of OH-is the highest. The content of flavonoids, total phenols and total anthocyanins in 6 varieties of blueberry fruits were positively correlated with the clearance rates of DPPH free radical, ABTS+free radical and OH-free radical,and the correlation reached an extremely significant level(P<0.01). In summary, Biloxi have higher titratable acid content, stronger antioxidant capacity than other 5 varieties, and it's suitable for comprehensive processing and high value utilization.

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张瑜瑜,宋怀飞,刘佳妮,姚丽媛,华金珠.云南主栽蓝莓果实可滴定酸及抗氧化活性评价研究[J].东北农业科学,2023,48(1):124-128,135.

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  • 收稿日期:2020-02-12
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  • 在线发布日期: 2024-10-24
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