To further improve the cultivation techniques of strong-flavor type tobacco with high quality, a two-factor split plot experiment was carried out in Yongan, Liuyang. The interaction effects of nitrogen application rate and planting density on population transmittance, yield and quality of flue-cured tobacco variety G80 were studied. The results showed that: The agronomic character of tobacco plant in treatment of N 240 kg/ha and low-density was better. Under the condition of the same density, the higher the nitrogen application rate, the higher the chlorophyll content and the lower the group transmittance. Under the condition of the same amount of nitrogen application, the light transmittance of low-density treatment was higher than that of high-density treatment. With the increase of nitrogen application rate, the yield and output value of flue-cured tobacco increased at first and then decreased. The output value of low-density treatment was the highest at the nitrogen application rate of 210 kg/ha, and the output value of high-density treatment was the highest at the nitrogen application rate of 180 kg/ha. Among the different density treatments, the economic characters of 16,500 plants/ha were preferable. The lower the amount of nitrogen application, the less nicotine content in flue-cured tobacco leaves, and the best sugar-alkali ratio and nitrogen-alkali ratio is 150 kg/ha. In conclusion, low nitrogen application and low planting density can improve the light transmittance of flue-cured tobacco, contribute to the photosynthesis of flue-cured tobacco, and improve the quality of flue-cured tobacco. The suitable nitrogen application rate for G80 in Liuyang was 180 kg/ha, and the suitable planting density was 16,500 plants/ha.