Abstract:ZIP protein is a kind of vital cation transporter, which plays an important physiological role in maintaining the cations dynamic equilibrium in the process of metabolism and growth development of organism. This study using the analysis of systematic bioinformatics method to clarify the sequence characteristics of SlZIP gene family and the expression pattern of those genes in different tissues and fruits at different development stages. The content includes the identification of SlZIP gene family members from tomato genome, and the analysis of its basic sequence characteristics, chromosome distribution, gene structure, transmembrane domain and tissue specific expression. The results showed that there were 15 members in SlZIP gene family and those genes were unevenly distributed on 7chromosomes, among the chromosome 2 with the largest number of SlZIP genes. The length of coding region, CDS sequence and amino acid sequence among the member of SlZIP genes have a large span difference. There were 9acidic proteins and 6 basic proteins in SlZIP, which were basically hydrophilic stable proteins. Gene structure analysis showed that 9 SlZIP genes had 3 exons and 2 introns. There were 5 and 4 SlZIP proteins with 8 and 9 transmembrane domains, respectively. And 13 SlZIP proteins possessed variable region which located on the inner membrane,and 7 SlZIP proteins possessed histidine rich domain which distributed in the variable region. Phylogenetic tree analysis indicated that the evolutionary relationship between SlZIP and AtZIP(Arabidopsis thaliana ZIP) was the closest. The study on tissue expression pattern showed that 7 SlZIP genes expressed in all tissues, and 12 SlZIP genes expressed in the roots. From the perspective of fruit expression, 11 SlZIP genes expressed in the fruits, but with significant differences expression in various fruit development stages. This study would provide an theoretical basis on cloning and functional research of SlZIP genes, and provide an theoretical foundation on through regulating SlZIP gene expression to fortify the Zn and Fe concentration in tomato fruits.