Phytoendophytes are important microbial resources. Isolation and identification of phytoendophytes provide an important base for the utilization of beneficial plant microbes. In order to preliminarily judge the phylogenetic relationship of endophytic bacteria in industrial hemp and further understand its biodiversity, using the traditional isolation and culture method, we isolated six bacteria strains from the leaves of an industrial hemp variety from Heilongjiang Province. The morphological, Gram coloring, 16s rDNA sequences and phylogenetic analysis were carried out. The morphology of the six strains were spherical. Five of them were Gram-positive bacteria, and one was Gram-negative bacterium. 16S rDNA BLAST result indicated that five strains were Bacillus, with 99.85%-100% similarity; and the other one was Acinetobacter, with 99.93% maximum similarity. By constructing phylogenetic tree, the six endophytic bacteria were obviously aggregated into two branches. The result can provide references for the exploitation and utilization of endophitic bacteria in Cannabis sativa.