Southern corn rust has been widespread and heavily emphasized in southern breeding bases in Hainan Province, which has caused a great damage to maize breeding in recent years. In this study, field treatment experiments in field were conducted on 20 treatments of 5 kinds of pesticides（25% pyrazoxystrobin, 19% propiconazoleazoxystrobin, 40% syringandrol-tebuconazole, 25% azoxystrobin and 0.01% brassinolide） to control the occurrence of southern corn rust. Two pesticide combinations were screened out from mixture both 40% syringandroltebuconazole and 0.01% brassinolide and mixture both 25% pyrazoxystrobin and 0.01% brassinolide. Spraying one time had a good effect on southern rust, with a control effect of 86.52% and long duration. The results showed that the use of appropriate combination of pesticides in the critical period can effectively control the outbreak of southern corn rust, and can eliminate the loss caused by southern corn rust on the maize breeding in southern breeding bases.