Abstract:Kaixuan 016 is a backbone parent for breeding high oleic acid peanuts in China, which is induced by introducing excellent foreign germplasm resources and has been used by many scientific research breeding units. In order to simplify the program of genetic diversity, thirty high oleic acid varieties derived from Kaixuan 016 were selected as an example, and an automated platform based on pedigree to calculate coefficient of parentage(COP) and cluster analysis was built using R language. Among the thirty derivatives, 70% of them had a COP of 0.5 with Kaixuan 016, and the other 30% had a COP of 0.25. The automatic platform showed the range of COP between the derivatives was from 0 to 0.75, with an average of 0.234. Cluster analysis distributed thirty varieties into six groups and the varieties bred by the same unit were likely clustered into one category. In conclusion, Kaixuan 016 has played an important role in improving the quality of peanuts in China. The automated platform for COP and clustering accelerated the process of genetic diversity analysis and provided an important reference for breeding new varieties.