The endophytic bacteria with antibacterial activity were screened with Astragalus membranceus as test material. Through the separation and purification of endophytic bacteria in roots, stems and leaves, and to examine their suppressive effects on three kinds of pathogenic fungi commonly found in fruit trees by using a pairwise competition experiment. The results showed that, 12 strains were isolated from astraglus membranceus. The effective action on C.diplodiella of 12 strains was 100%, while the effection on B.dothidea and A.alternata was 91.7%. Four strains（J₁, J₂, G₂ and Y₁） were screened from the agtagonism test between endophytic bacteria and B.dothidea, A.alternata, C.diplodiella. The differences between the effects of the four bacterial strains were not significant. Among these, strain J1exhibited the strongest antagonistic effect on the mycelial growth of the three pathogens at 72.87%,59.75% and 66.98%, respectively.