In recent years, the occurrence of wheat root rot has been increasing gradually. In order to reduce the losses caused by wheat root rot in production, this study compared the inhibitory effects of 8 fungicides for Bipolaris sorokiniana causing wheat root rot by laboratory toxicity test. The results of indoor toxicity test showed that epoxiconazole, difenoconazoleand·azoxystrobin and myclobutanil had a large inhibitory effect on wheat root rot, the EC₅₀were 0.005, 0.061, 0.462 mg/L, respectively, and EC₉₀were 0.349, 8.520, 7.782 mg/L, respectively. Resistance of the 56 wheat varieties from Hebei Province was identified by pot experiment. Kenong 2009 was the resistant variety,Jimai 22, Shannong 20, Guan 35, Shijiazhuang 8 and Zhoumai 22 were medium resistant varieties, the others were susceptible varieties and high susceptible varieties. The screening of effective fungicides and resistant varieties of wheat root rot laid a foundation for the prevention and control of wheat root rot disease.