This study investigates the impact of various hormone proportions on the growth of strawberry somaclones,specifically using the Hongyan and Fengxiang strawberry cultivars as test subjects. The analysis entailed using the tips of their stolons as explants and employing MS as the principal culture medium. The results showed that, the best bud induction medium for Hongyan was MS+6-BA 1.5 mg/L+KT 1.5 mg/L, the best bud induction medium for Fengxiang was MS+BA 1.5 mg/L+KT 0.5 mg/L. The subculture proliferation medium suitable for Hongyan is MS+6-BA 1.0 mg/L+NAA 0.4 mg/L, the multiplication factor can reach 12.3. The proliferation medium suitable for Fengxiang is MS+6-BA 1.0 mg/L+IBA 0.1 mg/L and the multiplication factor can reach 18.8. The suitable rooting medium for Hongyan is 1/2MS+IAA 0.5 mg/L, the suitable rooting medium for Fengxiang is 1/2MS+IAA 1.5 mg/L.