Abstract:In this study, eight bacterial strains, including Clavibacter michiganensis subsp. michiganensis(Cmm),were used as test materials, with the specific gene tom A as the detection target. LAMP primers tom A-107 and tom A-8, as well as the corresponding loop primers tom A-107LF21 and tom A-107LB21, were designed. The primer specificity and sensitivity tests were conducted for the target gene, and the results showed that primer tom A-107could specifically detect Cmm. The detection sensitivity of primer tom A-107 reached 5×102copies/μL for Cmm.The addition of loop primers shortened the reaction process by one third, enabling the entire detection process to be completed within 50 minutes. This method is highly suitable for rapid on-site detection of Cmm bacterial strains.