番茄溃疡病菌LAMP可视化检测方法的建立
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S436.412.1;S432.4+2

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吉林省重点科技攻关项目(20140204032NY)


Establishment of a LAMP Method for Detection of Clavibacter michiganensis subsp. michiganensis
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    摘要:

    以番茄细菌性溃疡病菌等7株病原细菌为检测材料,以特异性基因tom A为检测靶标,设计LAMP引物tom A-107、tom A-8和相应的环引物tom A-107LF21、tom A-107LB21。针对靶标基因进行引物筛选及引物的特异性和灵敏度测试,结果表明:tom A-107能特异性检测出番茄细菌性溃疡病菌,检测灵敏度达5×102copy/μL,环引物的加成将反应进程缩短三分之一,使整个检测过程控制在50 min内完成,该方法非常适用于Cmm菌株的现场快速检测。

    Abstract:

    In this study, eight bacterial strains, including Clavibacter michiganensis subsp. michiganensis(Cmm),were used as test materials, with the specific gene tom A as the detection target. LAMP primers tom A-107 and tom A-8, as well as the corresponding loop primers tom A-107LF21 and tom A-107LB21, were designed. The primer specificity and sensitivity tests were conducted for the target gene, and the results showed that primer tom A-107could specifically detect Cmm. The detection sensitivity of primer tom A-107 reached 5×102copies/μL for Cmm.The addition of loop primers shortened the reaction process by one third, enabling the entire detection process to be completed within 50 minutes. This method is highly suitable for rapid on-site detection of Cmm bacterial strains.

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刘燕妮,毛芙蓉,范惠冬,郑士金.番茄溃疡病菌LAMP可视化检测方法的建立[J].东北农业科学,2023,48(3):134-137.

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  • 收稿日期:2020-01-13
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  • 在线发布日期: 2024-10-24
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