Strain TK-2 was isolated from the topsoil of Nanzhang Wangguan Tea Garden in Xiangyang City, Hubei Province. The strain TK-2 was Gram-negative and long rod-shaped by microscopic examination. Its physiological and biochemical characteristics, as well as the 16S rDNA sequence characteristics, corresponded to the typical features of Pseudomonas aeruginosa. P. aeruginosa TK-2 was cultured in inorganic salt medium supplemented with fenvalerate for 72 h. The highest yield of fenvalerate degrading enzyme was obtained, with an enzyme activity of 10.8U/mL. After being purified by DEAE-52 and CM-52, the fenvalerate degrading enzyme of P. aeruginosa TK-2reached 3.4 times, and the recovery rate of enzyme activity reached 56.2%. The molecular weight of the enzyme was83.6 kDa after gel electrophoresis. The optimum temperature for degradation and purification of fenvalerate by P. aeruginosa TK-2 was 35℃, exhibiting good stability within the range of 25-45℃. The optimal pH value for the enzymatic reaction was 8.0, with good stability in the pH range of 7.0-9.0. The Michaelis constant（Km） for the substrate reaction was 0.738 mmol/L, and the maximum reaction rate Vmax was 1.123 mmol/（L·min）.