Jilin Province is rich in agricultural waste resources such as corn straw and peanut shell. This study explored the possibility and effectiveness of using corn straw and peanut shell as substrates for potato mini tuber production. Jishu 1, Youjin and Feiwuruita were used as experimental materials, and three kinds of substrate treatments（Vermiculite, corn straw, 1∶1 mixture of corn straw and peanut shell） were applied, and three density gradients（20,25, 30 plants/box） were set up. The results showed that the yield of micro tuber of Jishu 1 was the highest when the planting density was 25 plants/box and corn straw was used as the cultivation substrate. For Eugene variety, when the mixture of corn straw and peanut shell was 1∶1 as the substrate, the number of micro potato seeds was the largest under the density of 30 plants/box.