芜菁花叶病毒吉林分离物外壳蛋白基因克隆与序列分析
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S432.41

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吉林省重点研发计划项目(20210202131NC);国家自然科学基金项目(31201485)


Cloning and Aequence Analysis of cp Gene of Turnip mosaic virus Isolates in Jilin Province
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    摘要:

    为明确芜菁花叶病毒(Turnip mosaic virus, TuMV)在吉林省十字花科蔬菜上的侵染情况,用RT-PCR方法扩增获得吉林省16个TuMV病毒分离物的cp基因,并对其序列进行分析。结果表明,本研究所获得的16个吉林省TuMV分离物和2个山东省分离物cp基因序列同源性为90.3%~100%,与其他30个从GenBank上获得的TuMV分离物核苷酸同源性为81.9%~99.3%。TuMVcp基因系统进化分析显示:48个TuMV分离物分布在5个组,吉林省TuMV分离物存在basal-B组和word-B组2个株系,basal-BR组为优势株系,在Asian-BR、basal-B和OMS组中均无分布。重组分析表明,吉林省16个TuMV病毒分离物cp基因都未发生重组事件。选择压力分析显示cp基因编码区处于负向或纯化选择压力。

    Abstract:

    In order to identify Turnip mosaic virus(TuMV) infection in cruciferous vegetables in Jilin province, 16TuMV virus isolates cp genes were cloned. Sequence analysis results showed that the cp gene sequence identities of16 TuMV isolates from Jilin province and 2 isolates from Shandong Province obtained in this study was 90.3%-100%, and the nucleotide identities of the 16 TuMV isolates obtained in this study was 81.9%-99.3% compared with other 30 TuMV isolates in GenBank. Phylogenetic analysis showed that 48 of the TuMV isolates including 16isolates in Jilin Province, could be divided into 5 lineages. TuMV isolates in Jilin province were divided into 2 lineages corresponding to basal-B and word-B. Basal-B was the dominant isolates. But there was no isolates divided into the Asian-BR, basal-B and OMS lineages. The recombinant analysis of cp gene coding region of 18 TuMV isolates obtained in this study found that no recombinant phenomenon occurred. The results of selective pressure analysis showed that cp coding region was under negative or purified selective pressure, in which the selection pressure of the isolates in Asian-BR lineage was low, and that of the isolates in World-B lineage was the highest.

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王凤婷,李琳,孙玥,祝富祥,乔凯彬,张祥辉,潘洪玉,刘金亮.芜菁花叶病毒吉林分离物外壳蛋白基因克隆与序列分析[J].东北农业科学,2023,48(5):71-75.

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  • 收稿日期:2020-10-30
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  • 在线发布日期: 2024-10-25
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