In order to identify Turnip mosaic virus（TuMV） infection in cruciferous vegetables in Jilin province, 16TuMV virus isolates cp genes were cloned. Sequence analysis results showed that the cp gene sequence identities of16 TuMV isolates from Jilin province and 2 isolates from Shandong Province obtained in this study was 90.3%-100%, and the nucleotide identities of the 16 TuMV isolates obtained in this study was 81.9%-99.3% compared with other 30 TuMV isolates in GenBank. Phylogenetic analysis showed that 48 of the TuMV isolates including 16isolates in Jilin Province, could be divided into 5 lineages. TuMV isolates in Jilin province were divided into 2 lineages corresponding to basal-B and word-B. Basal-B was the dominant isolates. But there was no isolates divided into the Asian-BR, basal-B and OMS lineages. The recombinant analysis of cp gene coding region of 18 TuMV isolates obtained in this study found that no recombinant phenomenon occurred. The results of selective pressure analysis showed that cp coding region was under negative or purified selective pressure, in which the selection pressure of the isolates in Asian-BR lineage was low, and that of the isolates in World-B lineage was the highest.