VOZ protein is a type of plant-specific transcription factor, which plays an important role in regulating plant flower development and responding to biotic and abiotic stresses. In this research, two SlVOZ genes were identified by scanning the tomato whole genome, and the nucleotide sequence and protein sequence were systematically and comprehensively analyzed using bioinformatics methods, and gene expression patterns were studied. Analysis of the basic physical and chemical properties of proteins shows that they are all acidic, unstable, hydrophilic and nonfat-soluble proteins. Gene structure analysis shows that the two SlVOZ genes are all gene structure patterns with 4exons and 3 introns. The different components of the protein secondary structure accounts for the same trend. From high to low, they are random coils, α helix, extended chain, and β sheet. Their tertiary structures of proteins all have similar spatial conformations. The amino acid sequence has the same 5 motifs, and the arrangement order of the motifs is also consistent with the multiple sequence alignment results. The phylogenetic tree shows that the two SlVOZs are the closest in evolutionary relationship, and they have completed differentiation before the differentiation of monocots and dicots. These two SlVOZ genes have certain expression in different tissue parts, indicating that they do not have tissue expression specificity, but the gene expression strength is significantly affected by the different developmental stages of the fruit. The results of this article will provide theoretical references for further research on the cloning and function of tomato SlVOZ genes.