In order to screened out potato cultivars that were adapt to Qinghai plateau region which with high altitude and were cold, and make full use of Qinghai plateau region the greenhouse in winter time. This research was the first time planted potatoes in greenhouse of Qinghai Province region which with high altitude and were cold, five potato cultivars were comprehensively evaluated of biological characteristics, yield, quality and other factors. The results showed that Minshu 1 of which the period of emergence and whole growth period were short, the rate of emergence was as high as 98%, growing luxuriantly, potato piece weight was the heaviest（360.36 g）, and potato piece of volume was the largest（13.92 cm×7.54 cm）, and the weight of large, medium potatoes were the heaviest（10.13 kg）,proportion of small potatoes was the lowest（2.87%）, of potato, the shape was oblong, skin was yellow and smooth,color of potato tuber flesh was light yellow, and potatobad was shallow. Yield（3,169.85 kg/667m²） was significantly higher than the other four cultivars, the starch content was the lowest, at 13.01%, Vitamin C content was the highest（37.56 mg/100g） and significantly higher than the other four cultivars. This study shows that Minshu 1 which with high yield and good commodity characteristics, can be spreaded in greenhouse of the high altitude and cold areas in Qinghai province.