Low-temperature and deep sowing are the main limiting factors for direct seeding of rice in cold region.In this study, 168 japonica rice resources in cold region were screened for cold tolerance at germination stage, the results showed that there were significant differences in cold tolerance at germination stage, which could be divided into 5 grades, of which 55.3% were grade 1 and grade 3. The seedling emergence rate of 35 materials with cold tolerance at germination stage was significantly different under low temperature and deep sowing. The seedling emergence rate decreased significantly with the increase of sowing depth and the decrease of temperature, when the temperature is 15 ℃ and the soil cover is 2 cm, the emergence rate of five materials, Longjing 63, Longqingdao 45,Longjing 29, Longjing 46 and Longqingdao 64, is more than 70%, which can be used as variety selection of direct seeding in cold area.