绿豆AsA-GSH循环对初花期低温的响应及烯效唑的缓解效应分析
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S522.01

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黑龙江省重点研发计划项目(GA21B009-014);国家食用豆产业技术体系项目(CARS08-G8)


AsA-GSH Cycle Response of Mung Bean to Low Temperature at Initial Flowering Stage and the Analysis of Mitigation Effect of Uniconazole
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    摘要:

    为探究初花期低温及预喷施烯效唑(S3307)处理对绿豆(Vigna radiata L.)叶片AsA-GSH循环和产量的影响,以绿丰5号和绿丰2号为材料进行盆栽试验。在绿豆始花期预喷施S3307,并进行低温(平均12℃)连续5 d的处理,对绿豆叶片活性氧类物质(ROS)积累量、AsA-GSH循环物质的含量、渗透调节物质含量和绿豆产量进行分析。结果表明,始花期低温引起绿豆叶片过氧化氢(H2O2)含量和丙二醛(MDA)含量显著增加,抗坏血酸(AsA)和谷胱甘肽(GSH)含量提高,可溶性糖含量和脯氨酸含量有所增加,低温处理1~5 d导致绿丰5号单株产量下降14.87%~48.14%、绿丰2号单株产量下降20.60%~56.18%。低温条件下,预喷施S3307能有效增加绿豆叶片可溶性糖含量,显著降低H2O2、MDA含量,显著提高AsA、氧化型抗坏血酸(DHA)、GSH、氧化型谷胱甘肽(GSSG)含量。喷施S3307,正常温度条件下,绿丰5号和绿丰2号单株产量分别提高4.70%和7.87%;低温条件下处理1~5 d,绿丰5号单株产量提高5.11%~12.54%、绿丰2号单株产量提高6.30%~32.91%。初花期低温影响绿豆叶片活性氧物质积累、AsA-GSH循环、渗透调节等生理功能;预喷施S3307可有效缓解冷害对绿豆生理和产量的影响,本研究可为绿豆抗低温生产提供理论依据和技术支持。

    Abstract:

    In order to investigate the effects of low temperature stress during the initial flowering stage and prespraying with uniconazole(S3307) on leaf AsA-GSH cycle and yield of mung bean(Vigna radiata), Lvfeng 5(L5)and Lvfeng 2(L2) were used as materials in this pot experiment. S3307 was pre-sprayed and treated with continuous low temperature(average 12 ℃) for 5 d and samples were taken every day to analyze the accumulation of reactive oxygen species(ROS), changes in substance content of AsA-GSH cycle, content of osmotic adjustment substances in mung bean leaves and yield. The results showed that the accumulation content of hydrogen peroxide(H2O2) and malondialdehyde(MDA) in mung bean leaves were significantly increased by chilling damage at the initial flowering stage, and the content of ascorbic acid and glutathione were significantly increased. The yield per plant of L5 was significantly decreased 14.87%-48.14%, and the yield of L2 was significantly decreased 20.60%-56.18%. S3307 has the effect of resisting low temperature stress at the initial flowering stage. Under low temperature conditions, prespraying S3307 can effectively increase the soluble sugar content of mung bean leaves, significantly reduce the H2O2 and MDA content, and significantly increase the contents of AsA, DHA and GSH, GSSG. Spraying S3307 has the regulatory effect of increasing the yield of mung bean. Under normal temperature conditions, L5 and L2 in a single plant significantly increased by 4.70% and 7.87%, respectively. Under low temperature conditions, when treated at low temperature for 1-5 d, the yield of single plant increased 5.11%-12.54%, and the yield per plant was significantly increased 6.30%-32.91%. In conclusion, low temperature during the initial flowering period affected the physiological functions of mung bean leaves such as reactive oxygen species accumulation, AsA-GSH cycle and osmoregulation. Pre-spraying S3307 could effectively alleviate the effects of low temperature stress on mung bean physiology and yield, and this conclusion provides theoretical basis and effective measures for the production of mung bean resistance to low temperature production.

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项洪涛,李琬,王雪扬,王德明,张晓青,王强,孟宪欣,尹振功,王晨,高嫱.绿豆AsA-GSH循环对初花期低温的响应及烯效唑的缓解效应分析[J].东北农业科学,2024,49(3):13-20.

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  • 收稿日期:2024-01-16
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  • 在线发布日期: 2024-10-11
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