Verticilliam dahliae Allergen Asp f2-like（VDAL） is a new kind of plant activator protein. After sprayed on leaves, it can activate immunologic mechanisim of plants, induce systemic defense,promote plant growth and development by regulating the signal transduction pathway of plant hormones including Calmodulin（CaM） and Lipoxygenase 2（LOX 2） etc..In this assay, different concentrations of VDAL was sprayed on benihoppe leaves,the effects of VDAL on yield and quality of strawberry were evaluated by investigating the parameters of strawberry including fruit number, weight and abnormal fruit number. The results showed that compared with the control, the number of strawberry fruits increased 10.64%, the yield increased 6.85%, the Vc content increased 29.66%, the soluble sugar content increased 21.04%, and the abnormal fruit rate decreased 7.13% by spraying 3,000 times dilution. For benihoppe production, spraying 3 times from full bloom stage with 20 days interval could increase fruit quantity and yield, increase Vc content and soluble sugar content, and reduce abnormal fruit ratio.