HPLC法测定工业大麻中大麻二酚和四氢大麻酚的含量
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O657.7+2

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吉林省科技发展计划项目(20220404009NC)


Determination of CBD and THC in Industrial Hemp by HPLC
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    摘要:

    本研究在优化前处理条件和色谱分离的基础上,建立了高效液相色谱法(HPLC)测定工业大麻中大麻二酚(CBD)和四氢大麻酚(THC)的检测方法。样品经优化提取剂、流动相及色谱条件,最终选择用95%乙醇提取,乙腈-水(68∶32,v/v)为流动相,采用C18色谱柱进行分离,柱温35℃,流速1.0 mL/min,进样体积10μL,检测波长220 nm。结果表明,该方法在0.02~0.10 mg/mL浓度范围内具有良好的线性关系,相关系数(r)均大于0.998。方法检出限(LOD,S/N=3)和定量限(LOQ,S/N=10)分别为:CBD的检出限为0.000 1 g/100 g、定量限为0.000 3 g/100 g;THC的检出限为0.000 6 g/100 g、定量限为0.001 8 g/100 g。在已知样品中加入0.5 mg/kg、50 mg/kg和200 mg/kg三个浓度水平的CBD和THC标准溶液,进行加标回收试验,CBD的回收率为92%~101%,THC的回收率为80%~102%。该方法准确性、灵敏性和稳定性良好,能够满足工业大麻中CBD和THC的检测要求。

    Abstract:

    Based on the optimization of pretreatment conditions and chromatographic separation, a Highperformance liquid chromatography HPLC method for the determination of cannabidiol(CBD) and Δ9-tetrahydrocannabinol(THC) in industrial hemp was developed. The samples were extracted with 95% ethanol, acetonitrilewater(68∶32, v/v) as the mobile phase, and separated by C18 column at 35 °C with a flow rate of 1.0 mL/min, the injection volume was 10 μL and the detection wavelength was 220 nm. The results show that the method has a good linear relationship in the range of 0.02-0.10 mg/mL, and the correlation coefficients(r) are all greater than 0.998.The detection limits(LOD, S/N=3) and quantitative limits(LOQ, S/N=10) were 0.0001 g/100 g and 0.0003 g/100 g for CBD, 0.0006 g/100 g and 0.0018 g/100 g for THC, respectively. The recovery rates of CBD and THC were 92%-101% and 80%-102%, respectively when the negative samples were added with 0.5 mg/kg, 50 mg/kg and 200mg/kg. The method has good accuracy, sensitivity and stability, and can meet the detection requirements of CBD and THC in industrial hemp.

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华晶忠,张鑫,王丹,李盛钰,魏春雁,刘笑笑. HPLC法测定工业大麻中大麻二酚和四氢大麻酚的含量[J].东北农业科学,2024,49(3):87-92.

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  • 收稿日期:2023-08-01
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  • 在线发布日期: 2024-10-11
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