In order to clarify the genetic diversity of phenotypic traits and major cannabinoids content in hemp germplasm resources for flower and leaf use, and to screen for hemp germplasm resources with excellent comprehensive traits, providing basic materials and theoretical basis for genetic improvement and new variety breeding of hemp resources. Using Shannon-Wiener’s and descriptive statistics, genetic diversity analysis was conducted on 2 quality traits and 9 quantitative traits of 33 germplasm resources of Shannon-Wiener’s hemp for flower and leaf use. Use correlation analysis to clarify the correlation between various traits, and use principal component analysis, membership function values, etc. to evaluate the germplasm resources of cannabis for flower and leaf use. Genetic diversity indices for two quality traits of 33 hemp germplasm resources tested were 0.68 and 0.90, and the 9 quantitative traits showed a good normal distribution with a coefficient of variation range from 13.80% to 144.41%. Among them, the coefficient of variation for branch number was the smallest, and the coefficient of variation for THC content was the largest. Evaluate the germplasm resources of hemp for flower and leaf use through membership function values, and Select 5 germplasm with excellent comprehensive traits. 33 tested hemp germplasm resources can all grow and develop normally in the Daqing area, with significant differences among them and rich genetic diversity.