Starting with the evaluation indexes of eco-tourism suitability, resource and environment carrying capacity and ecological vulnerability, this paper uses the method of GIS technology and hierarchical analysis to construct a comprehensive evaluation system of forest eco-tourism, and divides the forest eco-tourism functional areas in Genhe City, Inner Mongolia. The results show that the research area is divided into three functional areas: the restricted area of eco-tourism, the moderate area of eco-tourism and the development area of eco-tourism, and 5 functional sub-areas: fine line tourism area, wetland tourism area, forest health tourism area, folk customs tourism area and original ecological tourism area. Both functional areas have their main functions and ecological landscape. Reasonable tourism regionalization can provide theoretical support for the in-depth development of ecotourism.