To optimize the test conditions of pork TPA analysis by response surface method. Texture analyzer was used to texture profile analysis of pork, and the influence of three test parameters such as test speed, time interval between two depressions, compression ratio on the results of pork texture properties was explored by single factor investigation. On the basis of single factor investigation, response surface methodology with three factors at three levels was adopted to optimize the test conditions of pork TPA analysis. The optimal test conditions were determined by the regression analysis. Test speed had significant effect on cohesiveness、springiness of pork (P<0.05), time interval between two depressions had significant effect on cohesiveness、stickiness、chewiness、hardness of pork (P<0.05), compression ratio had significant effect on cohesiveness、springiness、stickiness、chewiness、hardness of pork (P<0.05), test speed and time interval between two depressions interaction had significant effect on hardness of pork (P<0.05), test speed, time interval between two depressions, compression ratio do not hold up to the established model. Box-Behnken design was used to investigate the effects of optimum test conditions for pork TPA analysis were as follows: test speed of 1.42 mm/s, time interval between two depressions of 5.31 s, compression ratio of 50.1%.