Aiming at the production problems such as large area of greenhouse in rice seedling stage and difficult to take soil from seedbed soil, we discussed the impact of rice dense seedling mechanical insertion cultivation technology on its yield and economic benefits, so as to provide a theoretical basis for the application of rice dense seedling mechanical insertion cultivation technology. The medium-maturity variety "Jijing 305" was used as the test material, and the field test was conducted to analyze the effects of different seeding rate treatments on rice yield and economic benefits by setting three seeding rate levels, namely, low seeding rate treatment（CK）, medium seeding rate treatment（B₁）, and high seeding rate treatment（B₂）. The results showed that under the same fertilizer and water management conditions, there was no significant difference in the harvested yield of rice in the B1 treatment of dense seeding machine insertion compared with that of conventional machine inserted rice, but the production cost was lower than that of conventional cultivation technology inputs.