转基因动物技术及其应用
CSTR:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

S814.8

基金项目:

吉林省农业科学院基本科研经费项目(KYJF2023XK101)


Transgenic Animal Techniques and Applications
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    转基因动物技术是分子生物学前所未有的突破之一。转基因动物是一种携带其他物种基因或用于基因编辑的动物。动物特征可通过改变基因而有目的地改变。目的基因可以植入酵母人工染色体、细菌质粒和黏粒等多种载体中。热休克、电穿孔、病毒、基因枪、显微注射和脂质体转染都可以用来携带构建的质粒,进入宿主细胞。转基因技术可以在性腺、精子、受精卵中,利用DNA显微注射、逆转录病毒、干细胞和克隆技术来培育胚胎。目前最有效的转基因标记是荧光蛋白。转基因引发了一些伦理问题,本研究主要关注的是动物转基因的基本原理及其在工业、医药和农业中的应用。在解决社会和伦理问题后,未来转基因技术会更有前景。

    Abstract:

    Transgenic animal techniques is one of the unprecedented breakthroughs in molecular biology. A transgenic animal is one whose genome has been changed to carry genes from another species or to use techniques for animal genome editing for specific traits. Animal features can be changed by purposefully altering the genes. The produced gene of interest is placed into a variety of vectors, including yeast artificial chromosomes, bacterial plasmids and cosmids. Heat shock, electroporation, viruses, the gene gun, microinjection, and liposomes, are used to deliver the created vector, which includes the interesting gene, into the host cell. Transgenesis can be carried out in the gonads, sperm, fertilized eggs, and embryos through DNA microinjection, retroviruses, stem cells, and cloning. The most effective transgenic marker at the moment is fluorescent protein. Although transgenesis raises a number of ethical concerns, this review concentrates on the fundamentals of animal transgenesis and its usage in industry, medicine, and agriculture. If technology solves social and ethical problems, it will be the most promising in the future.

    参考文献
    相似文献
    引证文献
引用本文

李欣,刘昕昕,牛铭晨,赵玉民,曹阳,于力,赵中利.转基因动物技术及其应用[J].东北农业科学,2024,49(6):72-77.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2024-01-15
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2025-01-18
  • 出版日期:
文章二维码