Microbial reagents such as validamycin·bacillus subtilis 6%, plant growth regulator gibberellic acid·indol-3-ylacetic acid·brassinolide 0.136% and new type plant immune-activating proteins Verticillium dahliae Aspf2 Like 0.4% were selected. These three agents were respectively mixed with or replace conventional chemical agents, and used for seed-dressing of wheat and foliar spraying at two key growth stages. This study aims to clarify the field efficacy of this control method and the three biological agents against wheat crown rot. During seeddressing: in Treatment 1, validamycin·bacillus subtilis 6% was mixed with imidacloprid 70% for seed dressing.In Treatments 2 and 3, gibberellic acid·indol-3-ylacetic acid·brassinolide 0.136% and Verticillium dahliae Asp-f2Like 0.4% were respectively mixed with the conventional agent tebuconazole·imidacloprid 32% for seed dressing.At the seedling stage, the fresh weight of the underground part per plant in Treatment 1 was the largest, the plant height in Treatment 2 was the highest, and the fresh weight of the above-ground part per plant in Treatment 3 was the highest, which were 0.37 g, 29.35 cm, and 4.39 g respectively. All three were significantly higher than those of the conventional agent treatment and the blank control. Same agents were sprayed twice at the jointing stage and the flowering stage. In Treatment 1, only validamycin·bacillus subtilis 6% was used. In Treatments 2 and 3, gibberellic acid·indol-3-ylacetic acid·brassinolide 0.136% and Verticillium dahliae Asp-f2 Like 0.4% were respectively mixed with the conventional agent pyracostrobin· tebuconazole 30% for spraying. At the filling stage, the control effect was the highest in Treatment 3, followed by Treatments 2 and 1, which were 73.80%, 69.52% and 68.99% respectively, significantly higher than that of the conventional agent treatment. The yield increase rate was also the highest in Treatment 3, followed by Treatments 2 and 1, which were 23.86%, 23.12% and 21.69% respectively.Three treatments all had the effects of promoting growth and increasing yield on wheat, and had a good control effect on wheat crown rot. Among them, the mixture of Verticillium dahliae Asp-f2 Like 0.4% and the conventional agent performed the best in terms of control effect and yield, and has a good application and popularization prospect in the prevention and control of wheat basal rot.