Tomato（Solanum lycopersicum） is a dual-purpose crop for fruit and vegetable, and its yield and quality formation is closely related to each cultivation factor. As we all know, the fine seed and fine cultivation techniques are key factors for high quality and yield formation in tomatoes. Based on this, "Tomato Shangjiao 2" was chosen to engage in the optimization of cultivation condition. In this study, four cultivation factors with different levels were designed, and based on the research results derived from yield and quality, we determined an optimized cultivation technology system for "Tomato Shangjiao 2" : five truss pinching, sowing on February 14th（Shanghai）, planting density of 4.08 plants/m², and fertilization levels of 1 500 kg/667 m² earthworm fertilizer and 7.5 kg/667 m² Trichoderma fastidiosa. This study confirmed the optimized cultivation condition of "Tomato Shangjiao 2", also provide theory reference for cultivation of small tomatoes high yield and quality.