Abstract:A new method of developing Sunflower cytoplasmic male sterile (cms) line was reported in this paper. Hybrid on the basis of Cms line was used as germplasm source to develop B-line and as Cms source to develop Cms line. First of all, three-way hybrids were obtained by crossing the hybrid with a maintainer, and then selection for selling was made to get recurrent parent plants which was backcrossed with Cms plants segregating from corresponding F2 line, untill the progeny was genetically similar to the recurrent parent except that it was male sterile. The results showed that segregating ratio of fertility of backerossing pro-genies in A-line fitted the theoretical distribution of a single dominant gene. All plants in A-strains were sterile in testing cross progenies, and fertility segregation did not occure in su-bsequent backcrossing progenies. This method can be used to de-velop new Cms Iines, it provided a new way to use hybrids as ger-mplasms. It can be used to increase seed set, oil content, and resistance to diseases of the breeding materials.