The callcus cultures of somatic cell tissues from matur embryos and juvenile spikes （0.5~1.0 cm） of 30 rice varieties with different cold resistance were examined. The results showed that different genotypical callus had obvious difference in callus growth rate and Plantlet regenerative rate. And the differences relatively stabilized in continuous culturing. The Plantlet regenerative rates of calli from mature embryo were lower and not correlated with cold resistance of those varieties at 15℃.But plantlet regenerative rates of calli from jurenile spikes were higher and the positive correlation between callus growth rate and cold resistance of those rice varieties were significant. Thus, the callus growth rates of callus cultures developed at 15℃ for 25 days present as a morphological index to determine cold resistance of rice varieties and this might be suggested as a way for determining cold resistance of rice varieties.