This paper reported hypocotyl protoplasts from sainfoin（Onobrychis Viciaefolia Scop） to form embrynic cell colonies. Protoplasts isolation of green hy pocotyl and cotyledon and yellow hypocotyl and cotyledon from aseptic seedling of sainfoin was achieved in an enzyme solution consisting of 1％ Hemi—cellulase HP 150, 0.4％ Cellulase Onozuka R—10, 0.1％ Pectolyase Y—23, cpw9M, within 16—18hr., at dark, 28℃, 15rpm. Yield of protoplasts from yellow hypocotyl was the hightest. Then the protoplasts isolafed were cultured in the protoplasts culture medium K8P. After 7 days of culturing, fresh medium with a siightiy lower csmolality was added. The protoplasts went through first division after 3 days of culturing, the division rate was 20—30％. The Protoplasts divided and developed into globular embryo after 15 days of culturing.