The dry seeds of 2 wheat varieties were irradiated with 5 doses of gamma ray. The leaves of M₁ seedling were asepticaly excised as explantsand in vitro cultured. The results indicated:5KR treatment could increase callus induction frequency(CIF) and callus growth rate (CGR); 20KR treatment also increase CGR but decrease CIF; While both 5 KR and 20KR treatments could stimulate plantlet regeneration, the latter appeared more effective.