Analytical results are reported in this pepar on Peroxidase isozyme, malic dehydrogenase isozyme and glutamate dehydrogenase isozyme in Lycopersicon esculenturn var. Marglobe ovaria pollened and prevented pollenation by means of continuous concentration gradient polyacrylamide gel electrophoresis and spectrophotometer. The results indicate that malic dehydrogenase isozyme and glutamate dehydrogenase isozyme activities rise and new isozyme bands appear in pollened ovaria and peroxidase-isozyme activity rise and new isozyme bands appear and malic dehydroge-nase and glutamate dehydrogenase activities lower in prevented pollenation ovaria. In the last part of the pepar, relations between the three isozymesand pollenation, fertilization and senescence of tomato are discussedpreliminarily.