The nodule′s growth, nodule nitrogenase activities and the total nitrogen content of leaves were researched in soybean under different proportion of NO₃^--N and NH₄⁺-N fertilizer.The results indicated that seven proportion of NO₃^--N and NH₄⁺-N reduced fresh nodule weight and inhibited activity of nodule nitrogenase, but increased the total nitrogen content of leaves.The negative correlation between the total nitrogen content of leaves and fresh nodule weight, nodule nitrogenase activity were significant respectively.