To obtain aseptic soybean seedlings,wide soybean and cultivated soybean were asepticized using HgCl₂ and NaClO.The results showed that the wide soybean performed much higer resistance to antiseptic than cultivatied soybean.Secondly,the soaked seeds of cultivatied soybean which were treated by antiseptic can’t germinate or very few seeds able to germinate,that means the antiseptic hurted the seeds seriously.Thirdly,the seeds grow faster on the surface of solid MS medium than that the seeds in other positions.