This study aimed to investigate the association between the polymorphism of intron 5 of LIM homebox protein 8(LHX8) gene and reproductive traits in Songliao black pigs. A total of 120 multiparous Songliao Black sows were genotyped for single nucleotide polymorphisms(SNPs) in intron 5 of LHX8 using Sanger direct sequencing, and the correlations between SNPs and reproductive traits were analyzed via SPSS 22.0. A T/C mutation was identified in intron 5 of LHX8, with three genotypes(TT, TC, and CC) and two alleles(T and C) detected. The dominant genotype and allele were CC and C, respectively. Chi-square goodness-of-fit test showed that the SNP was in HardyWeinberg equilibrium and exhibited moderate polymorphism(0.25P<0.05). Birth weight was significantly higher in the TC genotype than in both TT and CC genotypes(P<0.01), with CC significantly higher than TT(P<0.05). These results indicate that the T0C mutation in intron 5 of LHX8 is a potential genetic locus associated with reproductive traits in Songliao black pigs. However, validation in larger populations is required to confirm its utility as a genetic marker for reproductive traits.